Question ID - 13871 | SaraNextGen Top Answer
Correct chronological order of the steps occurring during gene therapy are
I. Lymphocytes are obtained from the patients
II. Lymphocytes are transferred to the culture dishes
III. Lymphocytes are transfected with the normal ADA genes
IV. The transfected cell are returned to the patients
The chronological order should be
a)
I, II, III and IV
b)
II, I, III and IV
c)
I, III, II and IV
d)
III, II, IV and I
Correct chronological order of the steps occurring during gene therapy are
I. Lymphocytes are obtained from the patients
II. Lymphocytes are transferred to the culture dishes
III. Lymphocytes are transfected with the normal ADA genes
IV. The transfected cell are returned to the patients
The chronological order should be
a)
I, II, III and IV
b)
II, I, III and IV
c)
I, III, II and IV
d)
III, II, IV and I
(a)
(i) 
(ii) 
(iii) 
(iv).
Adenosine deaminase enzyme is very important for the immune system to function. In the absence of adenosine deaminase enzyme, purine metabolism is disturbed and T-lymphocytes fails to function. ADA deficiency can lead to Severe combiuned Immune Deficiency (SCID)
SCID is caused due to defect in the genes for the enzyme adenosine deaminase. The genetic diseases that are being investigated for gene therapy ranges from sickle-cell anaemia to Severe Combined Immuno Deficiency (SCID). In some children, ADA deficiency can be cured by bone marrow transplantation
However, in others it can be treated by the enzyme replacement therapy, in which functional ADA is given to the patient by injection. But in both approaches, the patients are not completely cured. For permanent cure, gene isolated from the bone marrow cells producing ADA at early embryonic stage can be a possible cure
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