Mycobacterium tuberculosis is having decimal reduction time of $13.5 \mathrm{~s}$ at $72^{\circ} \mathrm{C}$. The count of the organism is to be reduced by eight logarithmic cycles in milk pasteurization with adequate holding at a temperature of $85^{\circ} \mathrm{C}$. If $\mathrm{Q}_{10}$ value for the organism is $7.5,$ the minimum holding time for the desired sterility of this particular organism at $85^{\circ} \mathrm{C}$ is_______________ seconds.

Mycobacterium tuberculosis is having decimal reduction time of $13.5 \mathrm{~s}$ at $72^{\circ} \mathrm{C}$. The count of the organism is to be reduced by eight logarithmic cycles in milk pasteurization with adequate holding at a temperature of $85^{\circ} \mathrm{C}$. If $\mathrm{Q}_{10}$ value for the organism is $7.5,$ the minimum holding time for the desired sterility of this particular organism at $85^{\circ} \mathrm{C}$ is_______________ seconds.

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